Lana Darabkeh

Email: Lanadarabkeh@gmail.com

Location: Irbid-Jordan

CV:  Download here 

Thesis Title: Synergistic Anticancer Activity of the Heterogeneous TiO₂/Cu@Mn Nanocomposite Combined with Electromagnetic Radiation

Thesis Description:

Lana’s research investigates the development of Cu–Mn co-doped TiO₂ nanocomposites as potential radiosensitizers for enhancing anticancer therapy, particularly in pancreatic cancer. The nanoparticles were synthesized through a hydrothermal method at doping levels ranging from 0.5% to 5% and subjected to comprehensive physicochemical characterization. Analyses confirmed the presence of the anatase TiO₂ phase, successful incorporation of Cu and Mn dopants, increased agglomeration at higher doping ratios, a reduction in optical band gap, and changes in surface charge behavior.

Biological assessments revealed that the PEGylated 5% Cu–Mn co-doped TiO₂ formulation showed significant cytotoxicity toward PANC1 pancreatic cancer cells, with much lower IC₅₀ values compared to normal HDF fibroblast cells. Flow-cytometry analysis demonstrated that while pure TiO₂ primarily induced necrosis, combining the co-doped nanoparticles with X-ray electromagnetic radiation shifted PANC1 cell death from necrosis to apoptosis, confirming a strong radiosensitizing effect. In contrast, HDF cells displayed mostly necrotic responses, indicating differential cellular sensitivity.

Overall, Lana’s findings highlight the potential of Cu–Mn co-doped TiO₂ nanoparticles—especially at 5% doping with PEG coating—as promising radiosensitizers capable of enhancing apoptosis in pancreatic cancer cells. The work underscores the need for further optimization, improved targeting strategies, and in vivo validation to minimize off-target toxicity and improve therapeutic outcomes.
Status:

She graduated.